（Effect of APM against　UV Damage）

In recent years, ozone layer destruction has been increasing ultraviolet rays by an alarming degree. A consequent increase in the incidence of skin disorders is a social concern. The incidence of various skin disorders increases with increasing exposure to ultraviolet rays.Recent studies have shown that free radicals, which are produced in the skin tissue by the action of ultraviolet rays, are involved in the development of various kinds of skin disorders including skin cancer.

■The disorders of cell menbrane by UV-radiation

■Produce radicals by UV-radiation

Effect of APM against skin disorders by UV-radiation

(by kobayashi,1995)

In this experiments, the backs of mice were irradiated with ultraviolet B rays at an intensity of 15 kj/m2, mice were given 200 mg/kg of APM.

Also, immediately after irradiation by 2 kj of UV-B rays three a week,50 mg were administered, and this was continued for 25 weeks.

In this result, it is confirmed the increase in Malonyl Dialdehyde (MDA), which is an index for skin aging, and sialic acid ,which plays a role in inflammations .

■Effect of APM against skin inflammations by UV-radiation

APM is effective against skin disorders associated with irradiation by ultraviolet rays.

When the condition of the tumors that were produced on the backs of the mice were compared between the group of mice that were given APM and thegroup that were not, more mice had malignant tumors than in the untreated group, but in the treated group mice with malignant tumors were few and mice with benign tumors were larger in number. Moreover, the amount of MDA and sialic acid was low.The joint research group concludes from these results that it has been demonstrated that APM is effective in suppressing the damage done by ultraviolet rays.

■Effect of APM against skin tumors by UV-radiation

■Effect of APM against score of tumors no. by UV-radiation

Skin Tumors by UV-radiation

by kobayashi,1995

Micro scope photo. of skin tumors by UV-B

by kobayashi,1995

DIFFERENT SUSCEPTIBILITY OF EPIDERMAL KERATINOCYTES AND NEUROBLASTOMA CELLS TO CYTOTOXICITY OF UV-B IRRADIATION PREVENTED BY THE OXYGEN RADICAL-SCAVENGER APM BUT NOT BY ASCORBATE

(by MIWA, etal.,1995)

Reference: T.Kanatate,etal.,CELLULAR AND MOLECULAR BIOLOGY RESEARCH, Vol.41,No.6,pp.561-567,1995

■Mortality staining and cell size distribution of UV-B irradiated cells administered with APM

■Effects of Ascorbic acid derivatives on survival of UV irradiated cells.

■Cell survival ratio and relative fluorescence intensity of UV irradiated cells administered with APM

■Normal and fluoresent microphotographs taken for the same microscopic field of UV-B irradiated cells adoministered with APM.

■Preventive efffects of APM on cytolysis of UV-B irradiated cells.

■Efffects of APM on DNA dameges of UV-B irradiated cells.